https://doi.org/10.1140/epjp/i2019-12478-4
Regular Article
A simple method for labelling and detection of proteinaceous binders in art using fluorescent coumarin derivatives⋆
1
HERCULES Laboratory, Universidade of Évora, Largo Marquês de Marialva 8, 7000-809, Évora, Portugal
2
Chemistry Department, School of Sciences and Technology, Universidade of Évora, Rua Romão Ramalho 59, 7000-671, Évora, Portugal
3
CQE, School of Sciences and Technology, Universidade of Évora, Rua Romão Ramalho 59, 7000-671, Évora, Portugal
* e-mail: atc@uevora.pt
Received:
16
July
2018
Accepted:
17
December
2018
Published online:
20
February
2019
The identification of protein binders used in easel paintings is an important information to understand an artist’s technique and for making informed conservation and restoration decisions. The present work presents a novel fluorescent labelling methodology, using a coumarin derivative chromophore, C392STP (sodium (E/Z -4-(4-(2-(6,7-dimethoxy-coumarin-3-yl) vinyl) benzoyl)-2,3,5,6-tetrafluorobenzenesulfonate), as a fluorophore probe to bond proteinaceous binders used in paintings, followed by separation and identification with protein patterns, by electrophoretic processes. Additionally, theoretical quantum chemical calculations based on density functional theory (DFT) and time-dependent density functional theory (TDDFT) have been performed in a coumarin derivative, which mimics the coumarin bound to the proteins. The optimized methodology was tested on proteinaceous binder extracted from paint model micro-samples. The results evidence the applicability of this methodology as an effective and useful analytical tool for the identification of protein binders obtained from easel paintings.
© Società Italiana di Fisica and Springer-Verlag GmbH Germany, part of Springer Nature, 2019
