https://doi.org/10.1140/epjp/s13360-024-05357-y
Regular Article
High-resolution brain tractography from X-ray phase-contrast images
1
Université Claude Bernard Lyon 1, Lyon Neuroscience Research Center, CNRS, INSERM, 69500, Bron, France
2
LORIA, CNRS, Université de Lorraine, 54000, Nancy, France
3
Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, 63000, Clermont-Ferrand, France
4
Université de Strasbourg, CNRS, ICube UMR 7357, 67000, Strasbourg, France
Received:
31
January
2024
Accepted:
10
June
2024
Published online:
2
July
2024
X-ray phase contrast tomography (XPCT) can produce high contrast isotropic images of biological samples in only a few minutes thanks to the unique properties of the synchrotron X-ray beam. XPCT of brain tissue provides an exquisite contrast of white matter, which could be exploited for 3D directional analysis. In this article, we present the first XPCT-based pipeline to perform white matter tractography on rodent brains. We observed the disorganization of white matter in the vicinity of focal demyelinating lesions in the corpus callosum. The ability to obtain an isotropic, high-resolution tractogram is an important landmark which could drive further interest of the neuroscience community toward XPCT.
Matthieu Chourrout and Fabien Chauveau are Co-last authors.
Supplementary Information The online version contains supplementary material available at https://doi.org/10.1140/epjp/s13360-024-05357-y.
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© The Author(s), under exclusive licence to Società Italiana di Fisica and Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
