https://doi.org/10.1140/epjp/s13360-023-04340-3
Regular Article
An investigation of the efficacy of diagnostic X-ray radiation on bacterial survival
1
Department of Nuclear Physics, Science Faculty, Istanbul University, Istanbul, Turkey
2
Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey
Received:
19
April
2023
Accepted:
1
August
2023
Published online:
8
August
2023
The studies on the activity of routine diagnostic doses of radiation released from Roentgen and computed tomography on human microbiota are very limited. We aimed to investigate the in vitro effectiveness of X-rays used for diagnostic purposes on bacterial strains commonly found in human skin and intestinal microbiota. We used four standard bacterial strains: Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 700603 to represent human microbiota. All media were incubated at 37 °C for 24 h. The growing bacterial colonies were then exposed to radiation as desired in the protocols; CT and Roentgen imaging protocols were chosen in consideration of two separate approaches: from low energy to high energy and from a single exposure dose to multiple exposure doses. All media were reincubated at 37 °C for 24 h. The reduction percentages in colony numbers were calculated by comparing them with those in the control group, which has been treated in parallel but without exposure to radiation. The results of this investigation showed that this study revealed that X-ray doses of Roentgen were able to reduce only E. coli colonies that percentage ranged from 15 to 74%, whereas CT radiation doses had a negative effect on all bacterial species and vary between 0 and 49%. We found that, compared to Roentgen radiation, diagnostic CT radiation doses had a more thorough effect on bacterial species.
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© The Author(s), under exclusive licence to Società Italiana di Fisica and Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
