https://doi.org/10.1140/epjp/s13360-022-02933-y
Regular Article
Lens-less in-line holography through multiple-determined masks
Quantum Optics Lab., Department of Physics, COMSATS University Islamabad, Tarlai Kalan, Park Road, 45550, Islamabad, Pakistan
Received:
3
December
2021
Accepted:
8
June
2022
Published online:
22
June
2022
In this paper, a transmission mode optical lens-less system is required to achieve a super-resolved image. This technique is used for high resolution and large field of view. The multiple-determined masks are used to retrieve the amplitude and phase information of the sample. The setup consists of a coherent source followed by spatial light modulator, a USAF resolution chart, and a sensor in a column. The sample is illuminated with deterministic mask patterns in a sequential manner and encoded information of the sample propagated toward the sensor is recorded accordingly. The deterministic mask patterns are generated through ascending or descending order sorting from random distribution having pixel values of 
. The backward propagation model is applied to retrieve the sample information from the recorded raw images corresponding to each determined mask. A combination of phase and amplitude masks with determined modality is implemented. Primarily, simulations are used to demonstrate the efficacy of the optical system where peak signal-to-noise ratio and mean square error are used to evaluate the reconstruction quality. The system is then experimentally verified by reconstructing of high-resolution image of the sample. Lens-less microscopy technique provides a compact, stable, and cost-effective optical system. The use of such kind of method is most widely found in biomedical engineering for diagnostic purposes of infection.
© The Author(s), under exclusive licence to Società Italiana di Fisica and Springer-Verlag GmbH Germany, part of Springer Nature 2022
