https://doi.org/10.1140/epjp/s13360-021-02019-1
Regular Article
Determination of composition of mixed biological samples using laser-induced fluorescence and combined classification/regression models
1
DLR Institute of Technical Physics, Im Langen Grund 1, 74239, Hardthausen, Germany
2
Institute of Physical Chemistry, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
3
Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745, Jena, Germany
4
Department of Industrial Engineering, University of Rome Tor Vergata, Via del Politecnico 1, 00133, Rome, Italy
5
Department of Biomedicine and Prevention, University of Rome Tor Vergata, Via di Montpellier 1, 00133, Rome, Italy
Received:
13
April
2021
Accepted:
1
October
2021
Published online:
9
November
2021
Laser-induced fluorescence (LIF) provides the ability to distinguish organic materials by a fast and distant in situ analysis. When detecting the substances directly in the environment, e.g., in an aerosol cloud or on surfaces, additional fluorescence signals of other fluorophores occurring in the surrounding are expected to mix with the desired signal. We approached this problem with a simplified experimental design for an evaluation of classification algorithms. An upcoming question for enhanced identification capabilities is the case of mixed samples providing different signals from different fluorophores. For this work, mixtures of up to four common fluorophores (NADH, FAD, tryptophan and tyrosine) were measured by a dual-wavelength setup and spectrally analyzed. Classification and regression are conducted with neural networks and show an excellent performance in predicting the ratios of the selected ingredients.
© The Author(s) 2021
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