https://doi.org/10.1140/epjp/s13360-023-04378-3
Regular Article
Adaptive scans allow 3D-targeted laser dissection to probe the mechanics of cell sheets
1
Aix Marseille Univ, CNRS, Centrale Marseille, Institut Fresnel, Marseille, France
2
Aix Marseille Univ, CNRS, Centrale Marseille, Institut Fresnel, Turing Center for Living Systems, Marseille, France
Received:
21
April
2023
Accepted:
10
August
2023
Published online:
22
August
2023
The mechanical actuation of cells by active forces from the cytoskeleton drives tissue morphogenesis. To understand these forces, multicellular laser dissection has become an essential tool for severing tissue locally and inferring tension from the recoil of surrounding structures. However, conventional laser dissection is limited by 2D steering, which is inadequate for embryos and developing tissues that are intrinsically 3D structures. In this study, we introduce a flexible near-infrared (NIR) fs-pulsed laser-dissection system that allows for dissection trajectories to proceed in 3D and adapt to the curved surfaces of cell sheets, which are prominent structures in embryos. Trajectories are computed through an unsupervised search for the surface of interest. Using this technique, we demonstrate sectioning of multicellular domains on curved tissue, which was not possible with regular NIR laser scanning. We apply the developed strategy to map mechanical stresses in the imaginal disc of the developing Drosophila wing. Our targeted, adaptive scans can be used in other nonlinear processes, such as two-photon fluorescence imaging or optogenetics. Overall, this new laser-dissection system offers an innovative solution for studying complex 3D structures and their mechanical properties.
Supplementary Information The online version contains supplementary material available at https://doi.org/10.1140/epjp/s13360-023-04378-3.
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© The Author(s), under exclusive licence to Società Italiana di Fisica and Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
